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... DNA transfer, biofilm formation, and digested with BamHI and HindIII and then ligated with T4 DNA ligase (Fermentas, a laboratory manual,

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The linear pieces were ligated together using Stratagene's T4 Ligase DNA was harvested using Fermentas' Gene Jet Plasmid the AutoVaxID Operations Manual.

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Genotyping of Francisella tularensis XbaI, SmaI, BglII, and SacII (all from MBI Fermentas) and ligated to the adapter-linker sequences with T4 DNA ligase

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The linear pieces were ligated together using Stratagene's T4 Ligase DNA was harvested using Fermentas' Gene Jet Plasmid the AutoVaxID Operations Manual.

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... using T4 DNA ligase (Fermentas). following the Homogenous Mass Extend protocol described in the Mass ARRAY User's Manuals the ligase is T4 DNA ligase.

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... 0.5 uL pTAL vector DNA, 0.5 uL 10× T4 ligase buffer, 0.5 uL T4 ligase to make a final 5 uL reaction volume (Thermo Scientific/Fermentas) Tango buffer

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